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Seminario
"Super resolution optical fluorescence microscopy"
Relatore: Alberto Diaspro- Università di Genova e Dipartimento di Nanofisica, IIT, Genova
Note: Seminario di Dipartimento
Aula Newton
08 Maggio 2013 ore 16.30
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Abstract
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| Super resolution optical fluorescence microscopy
Alberto Diaspro
It is well known and established that, for the most popular imaging mode
in optical microscopy, i.e. fluorescence, the diffraction barrier does no
longer provide an unsurpassable limitation for resolution and localization
accuracy. Furthermore, the terms “super resolution” and "optical
nanoscopy", coined earlier, have been implemented in real far field
optical microscopes, nowadays available for everyone to use without
extreme complexity. Here, we will discuss targeted and stochastic readout
methods using both single and multiphoton excitation, in terms of
resolution and localization precision accuracy. Individual molecule
localization (IML) implemented within selective plane illumination
microscopy (SPIM) will be addressed towards 3D super resolution imaging in
thick biological samples. STED two-photon excitation microscopy will be
discussed reporting about the possibility of using a single wavelength
(SW) both for two-photon excitation and STED depletion by implementing a
SW-2PE-STED microscope. Particular attention will be given to the
intensity issues related to the depletion process. Direct writing
lithography at super resolution will be discussed along with the
characterization of new fluorescent reporter super-resolution oriented. A
further topic will be related to the coupling of STED and Atomic Force
Microscopy. So far, a variety of architectures will be outlined in regard
to specific applications demanding for nanoscale investigations.
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